Objective To explore the roles of allograft inflammatory factor-1 (AIF-1) and interleukin-1β(IL-1β) in the pathogenesis of ulcerative colitis. Methods Ninety BALB/c mice were randomly divided into three groups, each with 30 mices. In group 1, dextran sulphate sodium (DSS) was applied for modeling of ulcerative colitis, and E. coli was applied for modeling of bacterial enteritis in Group 2. Group 3 was set as the normal control. Peripheral blood samples and colon tissues of mice were collected for detection of AIF-1 and IL-1βexpression level by ELISA. Results (1) In group 1, AIF-1 expression levels were (0.134±0.048) ng/ml in peripheral blood and (0.462±0.117) ng/ml in colon tissues, which were significantly higher than the levels in group 2 [(1.425 ± 0.229) ng/ml in peripheral blood, (1.261±0.48) ng/ml in colon tissues], P<0.05. (2) There was no statistically significant difference in IL-1βexpression level between group 1 [(0.894 ± 0.173) ng/ml in peripheral blood, (1.261 ± 0.048) ng/ml in colon tissues] and group 2 [(0.087±0.021) in peripheral blood, (0.149±0.057) in colon tissues], P>0.05. (3) AIF-1 and IL-1βexpression levels in mice of bacterial enteritis or ulcerative colitis had no relevance (P>0.05). Conclusion AIF-1 might be involved in the specific pathogenesis of ulcerative colitis, and IL-1βmay not participate in the pathogenesis.%目的 探讨同种异体移植物炎症因子-1 (AIF-1)及白细胞介素-1β(IL-1β)在溃疡性结肠炎致病机制中的作用.方法 90只BALB/c小鼠随机分为三组,每组30只,第1组用葡聚糖硫酸钠(DSS)造模为溃疡性结肠炎,第2组用大肠杆菌造模为细菌性肠炎,第3组为正常对照,取得各组小鼠的外周血及结肠标本,用ELISA的方法检测AIF-1、IL-1β的表达,比较其在三组小鼠体内表达的差异.结果 (1)溃疡性结肠炎小鼠AIF-1表达水平分别为外周血中(0.134±0.048) ng/ml,结肠中(0.462±0.117) ng/ml,均较细菌性肠炎[外周血中(1.425±0.229) ng/ml、结肠中(1.261±0.48)] ng/ml高,各组间比较差异均有统计学意义(P<0.05);(2)溃疡性结肠炎小鼠IL-1β表达分别为外周血中(0.894 ± 0.173) ng/ml,结肠中(1.261 ± 0.048) ng/ml,与细菌性肠炎[外周血中(0.087 ± 0.021) ng/ml、结肠中(0.149±0.057) ng/ml]比较差异均无统计学意义(P>0.05);(3)溃疡性结肠炎及细菌性肠炎小鼠AIF-1与IL-1β表达水平无相关性(P>0.05).结论 AIF-1可能参与了溃疡性结肠炎的特异性致病机制,IL-1β可能未参与特异性致病机制.
展开▼
