HPV感染患者宫颈局部及外周血T细胞亚群和白介素变化研究

摘要

目的 探讨人乳头瘤状病毒感染(HPV)感染患者宫颈局部及外周血T细胞亚群、多项白介素的变化情况.方法 选取2013年1月至2015年3月于本院进行诊治的90例HPV感染患者为观察组,同时期同龄的90例健康妇女为对照组,检测并比较两组受试者的宫颈局部及外周血T细胞亚群、白介素(IL-2、IL-6、IL-8及IL-10)血清水平,另将观察组中不同病理检测结果患者的宫颈局部及外周血T细胞亚群、白介素检测结果进行比较.结果 观察组的宫颈局部T细胞亚群的CD4+、CD8+及CD25+分别为(32.37±3.18)%、(27.67±3.20)%及(6.28±1.14)%,CD4+水平低于对照组的(40.45±4.62)%,CD8+及CD25+水平则高于对照组的(22.52±2.34)%及(3.53±0.66)%,外周血T细胞亚群的CD3+、CD4+、CD4/CD8水平[(64.24 ± 5.15)%、(32.24 ± 3.31)%、(1.60 ± 0.12)],均低于对照组[(69.89 ± 5.43)%、(40.18±3.65)%、(21.21±2.53)],而CD8+[(25.94±2.78)%]则高于对照组[(1.89±0.15)%];血清IL-6、IL-8及IL-10水平分别为(7.03±0.75) pg/ml、(8.75±1.16) pg/ml及(21.31±2.57) pg/ml,其均高于对照组的(3.54±0.56) pg/ml、(4.21±0.45) pg/ml及(12.52±1.64) pg/ml,IL-2水平为(21.32±2.35) pg/ml,则低于对照组的(26.56±2.78) pg/ml,且不同病理检测结果患者的三个方面检测结果比较差异均有统计学意义(P<0.05).结论 HPV感染患者宫颈局部及外周血T细胞亚群、多项白介素变化较大,且不同病理患者间的差异也较为明显.%Objective To investigate and study the changes of cervical local and peripheral blood T cell sub-sets and various interleukins in the patients with human papillomavirus (HPV) infection. Methods Ninety patients with HPV infection in our hospital from January 2013 to March 2015 were selected as observation group, and 90 healthy women of corresponding age at the same time were selected as control group. Then the serum levels of cervical local and peripheral blood T cell subsets and interleukins (IL-2, IL-6, IL-8 and IL-10) were detected and compared between the two groups. The detection results of cervical local and peripheral blood T cell subsets and interleukins were also com-pared between patients with different pathological examination results in the observation group. Results The levels of cervical local T cell subsets (CD4+, CD8+, CD25+) were (32.37±3.18)%, (27.67±3.20)%, (6.28±1.14)%in the observation group and (40.45±4.62), (22.52±2.34)%, (3.53±0.66)%in the control group, respectively, with CD4+lower in the obser-vation group and CD8+and CD25+higher in the observation group. The peripheral blood T cell subsets (CD3+, CD4+, CD8+, CD4/CD8) were (64.24 ± 5.15)%, (32.24 ± 3.31)%, (25.94 ± 2.78)%, (1.60 ± 0.12) in the observation group and (69.89±5.43)%, (40.18±3.65)%, (1.89±0.15)%, (21.21±2.53) in the control group, respectively, with CD3+, CD4+, CD4/CD8 lower in the observation group and CD8+higherin the observation group. Serum IL-6,IL-8 and IL-10 levels were (7.03 ± 0.75) pg/ml, (8.75 ± 1.16) pg/ml, (21.31 ± 2.57) pg/ml in the observation group, which were all higher than (3.54 ± 0.56) pg/ml, (4.21 ± 0.45) pg/ml, (12.52 ± 1.64) pg/ml in the control group, while the IL-2 level in the observation group was lower than that in the control group [(21.32±2.35) pg/ml vs (26.56±2.78) pg/ml]. The differences in the index-es between patients with different pathological examination results in the observation group were statistically significant (P<0.05). Conclusion The change of cervical local and peripheral blood T cell subsets and various interleukins in pa-tients with HPV infection are significant, and the differences between patients with different pathological examination re-sults are also significant.