Objective To investigate the effect of Tongbi First Decoration(TFD)for treating rheumatoid arthritis (RA)on the serum concentrations of interleukin(IL)-18 and vascular endothelial growth factor(VEGF).Methods Fifty SPF Wistar rats were divided into normal group(n=10),model group(n=20),control group(n=10)and test group(n=10) by random number table.Rats in model group,control group and test group were treated with bovineⅡcollgen(BⅡC) and complete Freund's adjuvant (CFA) for establishment of RA rat models. Rats in test group were then treated with TFD,while those in control group were treated with tripterygium glycosides.Rats in model and normal group were treat-ed with isometric distilled water.The serum concentrations of IL-18 at different time points(1 d,7 d and 14 d after mod-eling) were tested by enzyme-linked immunosorbent assay (ELISA). Then the serum concentrations of IL-18 and VEGF before and after medication were tested by ELISA.Arthritis index and pathological score were collected for evalu-ation of pathological change.Results Before medication,the serum concentrations of IL-18 at 1 d,7 d and 14 d after modeling in model group were(24.68±3.67)pg/mL,(47.31±4.27)pg/mL,(70.91±6.13)pg/mL,showing a positive corre-lation with time(y=23.11x+1.40,R>0.99).After medication,the serum concentrations of VEGF and IL-18 were(86.47± 5.04)pg/mL,(34.83±2.72)pg/mL in test group,versus(101.56±7.36 pg/mL,(42.72±4.22)pg/mL in the control group (P<0.05). Moreover, after medication, the arthritis index and pathological score of test group was significantly lower than those in the control group:(5.50±0.85)vs(6.60±0.84),(1.30±0.48)vs(1.50±0.53),P<0.05.Conclusion Tongbi first decoration could inhibit the inflammatory development and the changing of knee joint in RA rats,which was related to the decrease of the overexpression of IL-18 and VEGF.%目的 研究通痹1号方对实验性类风湿关节炎(RA)大鼠血清白介素(IL)-18与血管内皮生长因子(VEGF)水平的影响.方法 选取50只清洁级Wistar大鼠,按随机数表法分为正常组10只、实验组10只、对照组10只与模型组20只.模型组、对照组和实验组使用牛Ⅱ型胶原-完全弗氏佐剂法建立Wistar大鼠RA动物模型.实验组予通痹1号方灌胃,对照组予雷公藤多苷灌胃;模型组与正常组均予等体积蒸馏水灌胃.酶联免疫法检测模型组(n=10)不同时间点(建模后1 d、7 d与14 d)血清IL-18的含量;酶联免疫法检测各组大鼠药物干预前后血清IL-18与VEGF的含量.使用关节炎指数与病理评分法评价各组大鼠药物干预后病理改变情况.结果 药物干预前,模型组RA大鼠血清IL-18建模后1 d、7 d、14 d分别为(24.68±3.67)pg/mL、(47.31±4.27)pg/mL、(70.91±6.13)pg/mL,呈浓度-时间正相关性(y=23.11x+1.40,R>0.99).药物干预后,实验组和对照组大鼠血清VEGF[(86.47±5.04)pg/mL vs (101.56±7.36)pg/mL]与IL-18[(34.83±2.72)pg/mL vs (42.72±4.22)pg/mL]水平比较,实验组明显低于对照组,差异均有统计学意义(P<0.05).此外,实验组药物干预后大鼠关节炎指数明显低于对照组[(5.50±0.85)分vs(6.60±0.84)分],差异有统计学意义(P<0.05);而且实验组大鼠病理学评分亦明显低于对照组[(1.30±0.48)分vs(1.50±0.53)分],差异有统计学意义(P<0.05).结论 通痹1号方能够抑制RA大鼠炎症进展与膝关节病变,其作用与减少RA大鼠IL-18的分泌和VEGF的过度表达相关.
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