真姬菇子实体ITS序列和RAPD分析

摘要

为真姬菇种质资源的评价和遗传育种提供分子水平的依据和基础,采用内转录间隔区(ITS)序列和RAPD技术分析了真姬菇不同子实体的遗传差异.结果表明:供试的5个白玉菇子实体和1个蟹味菇子实体ITS序列长度均为594bp,与已报道的真姬菇菌株ITS序列相似度为99％以上;供试的6个子实体间的遗传相似度变化范围为0.4123～0.6354;采用ITS方法能在物种水平上稳定地识别真姬菇,但不能有效区分白色白玉菇和褐色蟹味菇等不同真姬菇品系之间的差别.建议,对存在争议的真姬菇生产品种进行鉴定时,ITS分析可以先在种的水平上进行鉴定,种内不同品种(或品系)的鉴定需采用其他分子标记技术.%The genetic difference between different H. Marmoreus fruit bodies was determined by using ITS sequence and RAPD analysis method to evaluate its germplasm resources and to provide the molecular level basis for its genetics and breeding. The results showed that the ITS sequence length of five white H. Marmoreus and one H. Marmoreus was all 594bp and its sequence similarity to reported H. Marmoreus ITS sequence was above 99%. The genetic distance among six tested samples ranged from 0. 412 3 to 0. 635 4. ITS analysis could identify H. Marmoreus at the species level, but could not identify difference in different H. Marmoreus lines. Therefore, difference between different H. Marmoreus varieties (lines) should be identified by other molecular marker techniques.