绿茶中儿茶素的最佳提取条件研究

摘要

To obtain the optimum conditions of catechins extracted from green tea with ethanol,ethanol concentration,extraction temperature and extraction time were studied.The results showed that the ethanol concentration,extraction temperature,and extraction time affected extraction efficiency.The orthogonal experiment results showed that the optimal conditions for extraction and purification of catechins as follows： ethanol concentration was 75%,extraction temperature was 88.4 ℃,extraction time was 1.5 h,and the factors affecting the effect of catechins extract as follows： 〉extraction temperature〉extraction timeethanol concentration.%为了获得儿茶素乙醇提取工艺的最佳条件,主要研究了乙醇浓度、浸提温度和浸提时间对儿茶素乙醇提取效果的影响。依据儿茶素与香荚兰素的羟醛缩合生成红色产物的显色反应原理,以无机强酸为催化剂,用分光光度法对提取物中儿茶素含量进行测定。结果表明乙醇浓度、浸提温度、浸提时间对提取效果均有影响。正交试验结果表明,儿茶素的最佳提取和纯化条件为：乙醇浓度为75%,浸提温度为88.4℃,浸提时间为1.5 h,影响儿茶素提取效果的因素依次为：浸提温度〉浸提时间〉乙醇浓度。