利用火焰原子吸收光谱法对块菌酒中的锌元素含量进行测定,以锌元素含量为响应值,利用Design-expert8.0.6软件Box-Behnken中心组试验原理在单因素水平试验的基础上对块菌酒发酵工艺参数进行优化,其参数值为pH值为3.39；酵母菌添加量为1.4%；搅拌次数为3.19次/天,即每天搅拌次数为3次。根据最佳参数值进行三次重复发酵试验,测得锌元素含量的平均值为3.1372μg/mL,可见基于响应面优化的块菌酒中锌元素含量影响因素的最佳值较为可靠。%Zinc content in Truffle liquor was determined by using flame atomic absorption spectrometry, and box-behnken design of Design-expert 8. 0. 6 software was used to analyze the influence factors based on the single-factor tests, the results showed that pH value was 3. 39 , amount of yeast was 1. 4% and the stirring times was 3 times/day. After three repeated tests according to the optimum parameters, the average zinc content was 3. 1372μg/mL, showed the reliability of response surface method for determining the optimum values influenced by the zinc content.
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