高效液相色谱法测定复方新诺明的含量

摘要

Objective: A HPLC method was established for determination of Sulfamethoxazole compound.Methods:With a mobile phase of methanol－buffer (35:65), detection wavelength was 232 nm. Results: the average recovery of SMZwas 99.10% (RSD＝0.28%), the average recovery of TMPwas 97.89% (RSD＝0.46%). Conclusions:This HPLC method could be used for quality control of sulfamethoxazole compound.%目的：建立复方新诺明片含量测定的HPLC法。方法：以甲醇-缓冲液(35:65)为流动相，在232nm波长处检测。结果：复方新诺明中SMZ的平均回收率为99.10％，RSD＝0.28％；TMP的平均回收率为97.89％，RSD＝0.46％。结论：该方法简便快速，适用于复方新诺明片的含量测定。