一点红组织培养获得再生植株的研究

摘要

Objective To explore if the quick propagation of Emilia sonchifolia (L.) DC. in vitro culture could be used to provide tube-cultured seedlings in short time. Methods Axial buds were used as explants and cultivated on Murashige and Skoog basal medium by adding different portions of hormones. Results After 30-day cultured., axial buds which were cultivated in MS + BA1. 5mg· L + NAA0.1 mg· L-1 could induce fascicular buds, and each node derived 4 to 5 fascicular buds. The induced fascicular buds were transferred to the medium of 1/2 MS + NAA1 mg· L- 1 to induce roots. After the test-tube plantlet transplanted, there was no yellowing phenomenon (caused by yellow virus).Conclusion This research on tissue culture of Emilia sonchifolia (L.)DC. could supply tube-cultured seedlings for large-scale planting.%目的对一点红进行组织培养研究,探讨在短时间内快速繁殖一点红种苗的方法.方法以一点红带有腋芽的茎段为外植体,用MS+BA1.5 mg/L+NAA0.1mg/L培养基.结果经30 d诱导培养,可得丛生芽4～5个;小芽苗转入1/2MS+NAA1mg/L培养基上诱导生根,20 d后可长成具有3～4条根系的再生植株.结论此途径为人工栽培一点红提供优质种苗.