Study on callus induction and plant regeneration of Leuzea carthamoides via tissue culture system

摘要

Leuzea (Rhaponticum carthamoides) is a valuable medicinal plant from Asteraceae. Micropropagation could be a good alternative for the mass propagation of Leuzea carthamoides. To investigate the callogenesis of leaf explants, 12 different hormonal combinations including different concentrations of 16-benzylaminopurine (BA) and 2,4-dichlorophenoxyacetic acid (2, 4-D) were studied in two separable experiments. In both experiments, the explants were transferred to the Ms medium supplemented with 0.5 mg L-1 indole acetic acid (IAA) and 0.5 mg L-1 BA for 7 and 50 days after culture for regeneration, respectively. Then, after one month the percentages of callogenesis and the amount of produced callus were measured. In other experiment to investigated regeneration of root explants, 9 different hormonal combinations were studied including different concentrations of BA and IAA. The number of leaf per explants, length of greatest leaf per explant and regeneration percentage were measured one month after culture. The maximum callus production was obtained using 1 mg L-1 2, 4-D and 1.5 mg L-1 BA and 0.25 mg L-1 2, 4-D and 1.5 mg L-1 BA in first experiment and second experiment, respectively. In the third experiment, root explants had direct regeneration and medium with 0.5 mg L-1 BA + 1 mg L-1 IAA can be suitable medium.