method for the preparation of sorghum differentiation embryogenic callus tissue, method of producing a genetically modified sorghum cell, method of producing a genetically modified sorghum plant or regenerative part thereof, method of producing offspring of a sorghum plant genetically modified, genetically modified sorghum plant, offspring or part thereof produced using a method, culture medium suitable for use in the preparation of sorghum-differentiating embryogenic callus tissue and sorghum-differentiating embryogenic callus tissue

摘要

method for the preparation of sorghum differentiation embryogenic callus tissue, method of producing a genetically modified sorghum cell, method of producing a genetically modified sorghum plant or regenerative part thereof, method of producing offspring of a sorghum plant genetically modified sorghum plant, offspring or part thereof produced using a method, culture medium suitable for use in the preparation of sorghum differentiating embryogenic callus tissue and sorghum differentiating embryogenic callus tissue. to an improved method for sorghum transformation and genetically modified sorghum production. In particular, methods and media are described for the production of high quality transformable sorghum plant cells that are kept in culture longer without losing their regenerative potential and their use for genetic transformation. The method also describes the use of improved means for efficient plant regeneration from transformed plant cells, thereby providing significant improvement in stable sorghum transformation frequency.