A PROCESS OF PREPARATION OF TISSUE CULTURE MEDIUM FOR ENHANCING IN-VITRO PLANTLET REGENERATION IN AIR YAM PLANT USING BACTERIAL CULTURE SUPERNATANT.

摘要

This invention relates to a process of preparation of tissue culture medium for enhancing in-vitro plantlet regeneration in air yam plant using bacterial culture supernatant, in place of chemical growth promoter, comprising the steps of; growing Azotobacter chroococcum strain (A chroccoccum) in Jensen medium and/or Gluconacetobacter diazotrophicus strain (G. diazotorphicus) grown in LGI medium given by Covalcante and Dobereiner; adding to both media 75-125 mg/1 D,L tryptophan preferably 100 mg/1 as precursor of indoleacetic Acid (IAA); characterized in that centrifuging the bacterial culture after 4-8 days preferably 4 days for G. diazotrophicus medium and 8 days for A. chroococcum medium at 10,000 rpm for 18-25 minutes preferably 20 minutes to obtain Culture Supernatant; adding Culture Supernatant, obtained from step (ii), to Murashige and Skoog basal medium for nodal explants' growth; wherein Culture Supernatant supplemented medium regenerates healthy roots and shoots simultaneously in 25-30 days as compared to normal 40-45 days.