P53抗体在慢性 HBV 感染不同临床类型中的表达水平研究▲

摘要

目的比较HBV感染不同临床类型患者血清P53抗体水平，探讨其对肝癌的诊断价值。方法慢性HBV感染患者262例，其中慢性无症状乙肝病毒携带者51例（ ASC组）、慢性乙型肝炎51例（ CHB组）、乙型肝炎肝硬化52例（ LC组）、乙型肝炎结节型肝硬化53例（ HCN组）、乙肝相关性肝癌55例（ HCC组），应用酶联免疫吸附法（ ELISA ）检测血清P53抗体水平，并与50例健康体检者作对照（ NC组）。结果 NC组、ASC组、CHB组、LC组、HCN组P53抗体水平明显低于HCC组（P＜0．05）；HCC组患者P53抗体阳性10例（18．2％，10/55），其他各组均未检出P53抗体。血清P53抗体阳性与性别、年龄、民族、AFP定量、HBV-DNA拷贝数、血清白蛋白、血清胆红素、ALT/AST、有无肝硬化及有无肝外转移无关（ P＜0．05）。结论非恶变的乙肝病毒感染相关性肝病患者血清中无P53抗体表达，血清P53抗体表达可能与乙肝病毒感染相关性肝癌发生有密切关系。%Objective To compare the levels of serum P 53 antibody among patients with different clinical types of HBV infection ,and to explore the diagnostic value for hepatocellular carcinoma ( HCC ) .Methods Among 262 patients with chronic HBV infection ,there were 51 asymptomatic chronic hepatitis B virus carriers ( ACS group ) , 51 cases of chronic hepatitis B(CHB group),52 cases of hepatitis B with liver cirrhosis(LC group),53 cases of hepa-titis B with liver cirrhosis nodules ( HCN group ) , and 55 cases of hepatitis B-related HCC ( HCC group ) .Enzyme-linked immunosorbent assay (ELISA) was used to quantitatively detect the levels of serum P 53 antibody in the sub-jects,and 50 normal controls as NC group .Results The levels of P53 antibody in the NC group ,ASC group,CHB group,LC group and HCN group were significantly lower than that in the HCC group (P<0.05);There were 10 cases of positive P53 antibody in the HCC group (18.2%,10/55),no posive P53 antibody was found in the other groups . The positive rate of serum P53 antibody was not associated with gender ,age,nationality,alpha-fetoprotein(AFP), HBV-DNA,the levels of serum albumin,serum bilirubin and ALT/AST,liver cirrhosis and remote metastasis (P<0.05). Conclusion There is no expression of P 53 antibody in the serum of patients with non-malignant liver disease infected with HBV.The expression of serum P53 antibody may be associated closely with hepatitis B virus-related HCC.