宁夏回族人群血管紧张素Ⅱ2型受体基因C3123A多态性与原发性高血压的关系

摘要

目的 研究宁夏地区回族人群原发性高血压(essential hypertension,EH)与血管紧张素Ⅱ2型受体(angiogenesisⅡ type 2 receptor,AT2R)基因C3123A多态性的关系.方法 选取宁夏地区回族EH患者133例和血压正常者105例分别作为病例组(EH组)和对照组(NT组),应用聚合酶链反应-限制性片段长度多态性技术对以上人群的外周血白细胞DNA进行AT2R(C3123A)的基因多态性检测,以分析EH组和NT组基因型和等位基因的频率分布及其与EH的关系.结果 女性:EH组CC、AC、AA基因型频率(分别为32.9%、57.0%、10.1%)与NT组(分别为36.0%、60.0%和4.0%)相比差异无统计学意义(P>0.05),等位基因频率(C:61.4%;A:38.6%)与NT组(分别为66.0%和34.0%)相比差异无统计学意义(P>0.05);男性:等位基因频率(C:81.5%;A:18.5%)与NT组(分别为83.6%和16.4%)相比差异也无统计学意义(P>0.05).二元逐步logistic回归显示女性中体质指数、三酰甘油、肌酐增高为EH的危险因素,空腹血糖为保护因素;男性中体质指数、尿素氮增高为EH的危险因素,空腹血糖为保护因素.结论 AT2R基因C3123A多态位点分子变异与宁夏回族EH患者易感无明显相关性.%Objective To investigate the association between the polymorphism of angiogenesis Ⅱ type 2 receptor ( AT2R ) gene C3123A and essential hypertension ( EH ) in Hui population in Ningxia. Methods The genomic DNA was extracted from peripheral blood in 133 EH patients and 105 normal - tensive ( NT ) subjects in the Hui population in Ningxia. The C3123A point mutation of AT2R gene was detected by polymerase chain reaction - restriction fragment length polymorphism ( PCR - RFLP ), for analysis of the distribution of AT2R gene C3123A genotypes and alleles and the correlation between AT2R polymorphism and hypertension risk. Results In female subgroup: the genotype frequencies of CC, AC and AA were 32.9% , 57.0% and 10. 1% , respectively, in EH group; while those in normal group were 36.0% , 60. 0% and 4. 0% , respectively; with no significant difference between the 2 groups ( P >0. 05 ); the allelic frequencies of C and A were 61. 4% and 38. 6% in EH group, while they were 66. 0% and 34. 0% in normal group ( P >0. 05 ). In male subgroup: the allelic frequencies of C and A were 81. 5% and 18. 5% in EH group, while they were 83. 6% and 16. 4% in normal group ( P > 0. 05 ). According to binary stepwise logistic regression analysis, triglyceride, body mass index ( BMI ), creatinine were independent risk factors of EH, while glucose ( GLU ) as protective factor in famale subgroup; whereas BMI and blood urea nitrogen ( BUN ) were risk factors of EH, while GLU as protective factor in males. Conclusion There is no significant association between the C3123A polymorphism of AT2R gene and EH in Hui population in Ningxia.