Objective To evaluate the effects of atorvastatin on endothelial progenitor cells( EPCs ), and the spatial cognition and memory function in rats with traumatic brain injury ( TBI ). Methods Thirty - six adult male Wistar rats were randomly divided into sham group, treatment group and control group. Hydraulic combat was adopted to produce TBI in the treatment group and the control group, while sham surgical procedure without percussion injury was adopted in the sham group. Atorvastatin ( orally, 1 mg/kg ) treatment was given to the subjects in treatment group. Circulating EPCs were measured 7, 14 and 21 d after TBI. The mNss test was conducted 1,4,7, 14 and 21 d after TBI. The MWM during 21 ~25 d after TBI were also conducted, with the percentage of target quadrant recorded. Results Hydraulic combat impaired spatial cognition and memory function in rats. The EPC count was significant differed between the treatment group and the control group ( P <0. 05 ). The escape latencies were significantly higher in treatment group than that in control group on day 24 and day 25. Conclusion Atorvastatin can regulate circulating EPCs, improve spatial cognition and memory function in rats with TBI.%目的 观察阿托伐他汀对脑外伤大鼠内皮祖细胞(EPCs)、空间认知和记忆功能的影响.方法 选取36只成年雄性Wistar大鼠,随机分为假手术组、观察组和对照组,各12只.观察组和对照组采用液压打击法制作颅脑损伤模型,假手术组颅骨钻孔后不进行液压打击.观察组打击后1 h经口灌注阿托伐他汀 1 mg/(kg·d),连续14 d,对照组经口灌注等量生理盐水.术后第7、14、21天测量各组大鼠循环EPCs数量,术后第1、4、7、14、21天对各组大鼠神经功能进行mNss评分.术后第21～25天进行Morris水迷宫实验,记录大鼠目标象限百分率.结果 液压打击后大鼠的空间认知和记忆功能受损,大鼠mNss评分增高,经阿托伐他汀治疗后评分降低,第14、21天评分观察组与对照组差异有统计学意义(P<0.05).术后第7、14、21天,观察组EPCs数量较对照组显著增多,差异有统计学意义(P<0.05).术后第24、25天观察组大鼠逃避潜伏期较对照组显著缩短,差异有统计学意义(P<0.05).结论 阿托伐他汀能调节脑外伤后大鼠循环血EPCs数量,改善脑外伤大鼠空间认知和记忆功能.
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