Objective To evaluate the expression of serum cytokeratin 19 (CK19) and E-cadherin (E-cad) in various stages of oral carcinogenesis and investigate the correlation between CK 19 and E-cad in the process of oral cancer.Methods A total of 90 specific pathogen free ( SPF) male Wistar rats were randomly divided into two groups:4NQO-treated group (n=75,treatment of 40 parts per million [ppm] 4NQO solution) and healthy control group (n=15, treatment of water).The rats were sacrificed at different stages .The peripheral blood and tongue tissue samples were collected .The 5μm thick sections were stained with hematoxylin and eosin for histopathological analysis .Enzyme-linked immunosorbent assay ( ELISA) experimental method was used to study the expression of serum E -cad and CK19 in vari-ous stages of oral carcinogenesis .Pearson correlation coefficient and linear regression model were used to analyze the cor-relation between serum E -cad and CK19.Results The concentrations of CK19 and E-cad in normal group, epithelial hyperplasia group , mild dysplasia group , moderate-severe epithelial dysplasia group and squamous cell carcinoma group were (259.92 ±89.75) and (402.85 ±93.52) ng/L, (412.39 ±64.08) and (466.25 ±66.72) ng/L, (539.14 ± 108.07) and (593.45 ±134.06)ng/L, (784.45 ±108.53) and (848.58 ±99.68)ng/L, and (951.94 ±85.31) and (1 004.22 ±127.65)ng/L, respectively.There were significant differences between normal and epithelial hyperplasia , mild dysplasia, moderate-severe epithelial dysplasia or squamous cell carcinoma , in both E-cad and CK19 expression (P<0.05).The contents of CK19 and E-cad in peripheral blood were positively correlated (r=0.738, P<0.001). Conclusion The up-regulation expression of CK 19 and E-cad in peripheral blood is an early event in rat tongue muco-sa carcinogenesis .%目的：研究大鼠舌黏膜癌变过程中外周血的细胞角蛋白19（CK19）和E－钙黏蛋白（E－cad）的表达及相关性。方法90只SPF级SD雄性大鼠，按照随机数字表法分为6组，利用4NQO饮水法诱导75只大鼠（实验组）舌黏膜癌变，15只大鼠（对照组）饮用SPF级动物专用纯净灭菌水，于不同时期分批乙醚麻醉抽取外周血，颈椎脱臼处死取舌体组织做病理诊断，采用酶联免疫吸附试验（ ELISA）检测外周血中CK19和E－cad在大鼠舌黏膜癌变过程中的动态变化，Pearson相关系数及线性回归模型分析E－cad与CK19相关性。结果 E－cad和CK19在正常黏膜、上皮单纯增生、轻度上皮异常增生、中重度上皮异常增生和舌鳞状细胞癌组外周血中浓度分别为（259．92±89．75）、（402．85±93．52）、（412．39±64．08）、（466．25±66．72）、（539．14±108．07） ng／L和（593．45±134．06）、（784．45±108．53）、（848．58±99．68）、（951．94±85．31）、（1004．22±127．65） ng／L。上皮单纯增生、轻度上皮异常增生、中重度上皮异常增生和舌鳞状细胞癌组外周血中E－cad和CK19表达显著高于正常黏膜（ P＜0．05）；E－cad和CK19的表达呈正相关（r＝0．738， P＜0．001）。结论外周血中CK19和E－cad表达上升是大鼠舌黏膜癌变过程中的早期事件。
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