Objective To investigate the effects of electroacupuncture stimulation on analgesic for diabetic neuro -pathic pain in rats.Methods A total of 128 male adult SD rats were randomly divided into 4 groups (n=32):diabetic neuropathic pain model group ( Group D ) and electroacupuncture stimulation group ( Group EA ) , sham EA stimulation group ( Group E) , and sham acupuncture point group ( Group A) .The changes of mechanical shrinkage threshold , weight and blood glucose on the day before acupuncture stimulation and 7 days, 14 days, 21 days after electroacupuncture stimu-lation were recorded.Eight random rats from each group were sacrificed for L 4-6 spinal cord to inspect p38MAPK ex-pression levels in the spinal cord .The correlation between pain threshold and p 38 MAPK expression were analyzed .Re-sults Compared with Group D, E and A, significantly higher point thresholds were observed in Group EA at T 2, T3, and T4 (P<0.05).Compared with Group D, E and A, significantly p38 MAPK activation levels were observed in Group EA at T2, T3 and T4 (P<0.05).Pearson correlation coefficient for pain threshold and p 38 MAPK was -0.942.Conclu-sion Electroacupuncture can improve the pain threshold in DNP rats , the mechanism may be related to the inhibition of microglia p38MAPK expression in spinal cord .%目的：探讨电针刺激对糖尿病神经病理性疼痛大鼠痛阈的影响。方法128只雄性SD大鼠随机均分为4组（n＝32）：糖尿病神经病理性疼痛组（D组）造模成功后不给予治疗；电针刺激组（EA组）造模成功后给予电针治疗；假电针刺激组（A组）只将针灸针插入穴位，但不给予电刺激；假穴位电针刺激组（E组）将电针插入穴位右侧1 cm处，并给予电刺激，记录电针干涉前（T1）、电针干涉7 d（T2）、14 d（T3）及21 d（T4）大鼠的机械缩足反射阈值及血糖的水平；各时间点测定结束后，每组随机选取8只大鼠取L4－6脊髓，用Western blot的方法测定脊髓p38 MAPK表达水平，分析痛阈变化及p38 MAPK表达水平的相关性。结果与D、A、E组相比，EA组T2、T3、T4时间点痛阈均较高，差异有统计学意义（P＜0.05）；与D、A、E组相比，EA组T2、T3、T4时间点p38 MAPK活化水平均下降，差异有统计学意义（P＜0.05）；DNP大鼠痛阈及p38 MAPK变化呈负相关（r＝－0.942，P＜0.05）。结论电针刺激能提高糖尿病神经病理性疼痛大鼠的痛阈，其机制可能与抑制脊髓p38MAPK的表达有关。
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