目的 通过将固定浓度的念珠菌菌悬液分别滴加到含有不同浓度CaCl2的羊血培养基上培养,观察念珠菌溶血活性的差异,评估CaCl2对念珠菌溶血活性的影响.方法 选择25株念珠菌,其中包括21株临床分离株和4株标准株,配制4个不同浓度CaCl2的羊血培养基,分别为0%、0.5%、1%和2.5%(g/mL),于37℃、5%体积分数CO2培养.观察24 h、48 h、72 h的溶血反应并拍照、记录.结果 念珠菌在不同浓度CaCl2的羊血培养基中的溶血指数存在差异,差异具有统计学意义(F=24.929,P<0.001).其中不含CaCl2的溶血指数为2.749±0.103,含0.5%、1%和2.5%依次为2.247±0.079、2.013±0.092和2.150±0.066.结论 不同浓度的CaCl2会影响羊血培养基上念珠菌的溶血活性.%Objective To investigate the influence of different concentrations of CaCl2 on the hemolytic activity of candida strains. Methods A total of 25 candida strains were selected, including 21 candida strains recovered from clinical specimens, and 4 reference strains of C. albicans (ATCC 90028) (American Type Culture Collection, Manassas, VA, USA), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), and C. tropicalis (ATCC 13803) respectively. CaCl2 was added at concentrations of 0%, 0.5%, 1%and 2.5 g%(wt/vol). Plates were cultured at 37℃in a 5%CO2 atmosphere for 24-72 h, and the hemolytic index (Hi) was analyzed. Results Comparsions of the hemolytic indices among the groups treated with 0.5%CaCl2 (Hi=2.247 ± 0.079), 1%CaCl2 (Hi=2.013 ± 0.092), 2.5%CaCl2 (Hi=2.150 ± 0.066) and the control (Hi=2.749 ± 0.103) reached statistical significance (P<0.001). Conclusion CaCl2 may produce a de-crease activity in the hemolysis of candida species.
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