目的 观察滋肾通络方对MCAO大鼠脑组织脑源性神经营养因子(brain derived neurophic factor,BDNF)蛋白含量的变化,以探讨BDNF在脑缺血中的意义及滋肾通络方对缺血周围区神经细胞损伤修复的作用.方法 制作MCAO大鼠模型,随机分为假手术组、模型组、中药高剂量组、中药低剂量组和西药组.灌胃20天后,取大鼠脑组织,用免疫组织化学法检测各组BDNF的蛋白表达.结果 假手术组海马区几乎见不到BDNF阳性细胞表达;模型组切片染色增强,阳性细胞略有表达;西药组、中药低剂量组及高剂量组均见到切片染色强,BDNF阳性细胞强表达,尤以中药高剂量组阳性细胞表达最强.结论 BDNF与损伤后神经元的修复作用有关.滋肾通络方可通过调节BDNF的合成,促进神经细胞的存活和损伤修复,从而减轻缺血性脑损伤.%Objective To observe the influence of the nourishing kidneys and unblocking collaterals formula on the BDNF protein amount in MCAO rat s brain tissue and explore the significance of BDNF in cerebral ischemia and the effect of nourishing kidneys and unblocking collaterals formula on recovery of damaged neural cells in the peripheral area of ischemia. Methods MCAO rate model was randomly classified into pseudo-operation group, model group, high-dosage herb group, low-dosage herb group, and western medicine group. After administration of medicine for 20 days, remove the brain tissue from rats and measure the expression of BDNF protein in each group with immunohistochemical method. Results In pseudo-operation group, there was hardly expression of BDNF positive cell; in model group, there was the increased section staining, and little expression of positive cells; in western medicine group, low-dosage herb group, and high-dosage group, there were the increased section staining and strong expression of BDNF positive cells, especially in the high-dosage herb group, with the strongest expression of positive cells. Conclusions BDNF is closely related to the recovery of damaged neural cell. The nourishing kidneys and unblock collaterals formula can promote the survival and recovery of neural cell by regulating the synthesis of BDNF, therefore to reduce the damage of ischemic brain injury.
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