Objective To observe effects of Naotaifang on MMP-9, NF-κB and TIMP-1 after focal cerebral ischemia in rats. Methods The rats were randomly divided into sham operation group, model group, and Naotaifang low- (3 g/kg), medium- (9 g/kg), high- dose (27 g/kg) group. After 3 days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was made by middle cerebral artery occlusion (MCAO) with suture method. Following 3 days, the rats were treated with previous method. On the third day, hippocampal C2 region of ischemic tissue was detected by HE dyeing. And the contents of MMP-9, NF-κB and TIMP1 proteins in hippocampal C2 region were measured by immunohistochemical method. Results The number of normal brain cells in high dose group of Naotaifang was more than that of the model group, and only a few cells appeared nucleus pycnosis. The MMP-9 expression of all dose groups of Naotaifang were significantly decreased than model group (P<0.05). The NF-κB expression of high and medium dose groups of Naotaifang were significantly decreased (P<0.05). The TIMP1 expression of all dose groups of Naotaifang were significantly increased compared with sham operation group (P<0.05). Conclusion The mechanism of Naotaifang protecting blood brain barrier against injury of cerebral ischemia may be involved in ameliorating MMP, NF-κB and increasing TIMP1 expression.% 目的观察益气活血中药脑泰方对局灶性脑缺血大鼠海马C2区缺血组织基质金属蛋白酶9(MMP-9),核因子-κB(NF-κB)及基质金属蛋白酶抑制因子1(TIMP1)表达的影响,探讨其治疗局灶性脑缺血的机制。方法随机将SD大鼠分为假手术组、模型组及脑泰方低、中、高剂量组(3、9、27 g/kg)。各组大鼠灌胃给药连续3 d,每日1次,预处理后采用大脑中动脉栓塞法(MCAO)制备大鼠局灶性脑缺血模型,术后连续灌胃给药3 d。采用HE染色观察海马C2区细胞,免疫组化法检测海马C2区MMP-9、NF-κB及TIMP1表达。结果脑泰方高剂量组大鼠正常细胞较模型组明显增多,仅少数细胞出现核固缩样改变；脑泰方高、中、低剂量组MMP-9表达明显降低(P＜0.05)；脑泰方高、中剂量组NF-κB表达明显下降(P＜0.05)；脑泰方高、中、低剂量组TIMP1表达明显升高(P＜0.05)。结论脑泰方干预通过抑制NF-κB、降低MMP-9表达,增强TIMP1表达,保护血脑屏障,减轻脑水肿,对局灶性脑缺血导致的血脑屏障损伤有一定保护作用。
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