补阳还五精简方对局灶性脑缺血大鼠脑组织 APJ表达的影响

摘要

Objective To observe the effects of simplified recipe ofBuyang Huanwu Decoction on expression of APJ in the brain of local cerebral ischemia rats;To discuss its mechanism of action. MethodsFocal cerebral ischemia rat models were established by middle cerebral arterial occlusion. The adult rats were randomly divided into sham-operation group, model group,Buyang Huanwu Decoction group and simplified recipe ofBuyang Huanwu Decoction group. Administration groups were given relevant medicine for gavage. The expressions of APJ protein and APJ mRNA at different time points were detected by Western blot and RT-PCR.ResultsCompared with model group and sham-operation group, the expression of APJ protein and APJ mRNA at different time points in Buyang Huanwu Decoction group and simplified recipe ofBuyang Huanwu Decoction group significantly increased (P<0.05). The expression of APJ protein at different time points showed no difference between simplified recipe ofBuyang Huanwu Decoction group andBuyang Huanwu Decoction group;while the expression of APJ mRNA in simplified recipe ofBuyang Huanwu Decoction group was higher than Buyang Huanwu Decoction group (P<0.05).ConclusionSimplified recipe ofBuyang Huanwu Decoction plays a role in neural protection and restoration by promoting the expression of APJ in the brain of focal cerebral ischemia rats.%目的：观察补阳还五精简方对局灶性脑缺血大鼠脑组织APJ蛋白和基因表达的影响，并探讨其作用机制。方法采用大脑中动脉线栓法建立大鼠局灶性脑缺血模型，实验大鼠随机分为假手术组、模型组、补阳还五汤组和补阳还五精简方组，各给药组给予相应药物灌胃。分别于给药第3、7日Western blot、RT-PCR检测各组大鼠脑组织APJ蛋白和基因表达。结果补阳还五精简方组和补阳还五汤组各时间点APJ蛋白和基因表达均高于模型组和假手术组(P＜0.05)，补阳还五精简方组与补阳还五汤组各时间点APJ蛋白表达无明显差异，但补阳还五精简方组APJ基因表达高于补阳还五汤组(P＜0.05)。结论补阳还五精简方可通过促进缺血后脑组织APJ的表达发挥对大鼠脑组织的神经保护和修复作用。