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人参与胡萝卜体细胞杂交杂种愈伤的鉴定

             

摘要

Kinship relations of Panax ginseng are relatively far from Daucus carrot.Good traits of medicinal plants such like output qualith etc controlled by multiple gene through part of the gene transfer does not reflect all the material basis of the plants.Both of the parents to nuclear genes and cytoplasmic may be involved recombination in gene fusion events to propduce large amounts of a variety of individual genetic variation. Somatic hybrids between Panax ginseng and Daucus carrot by Electric fusion,screening for group of somatic cell hybrids,to obtain new germplasm. Foster innovation of variety achieve the purpose of improved species.We obtained 8 pieces of hybrid calli clone from the 25 calli clone systems after fusion, and identificated them through the morphology, cytology, isezyme, SDS-PAGE and other methods. We analyzed the index of physiological, biochemical and active ingredients of the hybrids, the results showed that the 8 hybrid calli contained Panax ginseng saponin, the contents of the 5 calli in them were higher than the ginseng calli, and the contents of 2 calli were 1.4 times of the ginseng calli. These indicated that the secondary metabolites contents of ginseng were increased by somatic cell hybridization of ginseng and carrot, the heterosis were reflected preliminarily.%贵重中药人参与胡萝卜亲缘关系较远,药用植物的优良性状如产量、品质等均由多基因控制,通过部分基因转移不能反映中药的全部物质基础.由于双亲的核基因和胞质基因都参与了融合事件,因而会发生核基因和胞质基因的重组,产生大量多样化的遗传变异个体,筛选获得的体细胞杂种群,可进一步获得我们所期望的新的种质资源,培育创新品种,达到改良物种的目的。用电容合法对人参与胡萝卜进行体细胞融合,对融合再生的25块杂种愈伤从形态学,细胞学,同工酶电泳,SDS-PAGE电泳进行鉴定分析。结果表明有8块为杂种愈伤无性系。测定生理生化指标与有效成分。结果表明获得的8个杂种愈伤组织无性系均含有皂苷.5个比人参含量高.说明人参与胡萝b体细胞杂种提高了人参次生代谢产物含量,体现了杂种优势。

著录项

  • 来源
    《人参研究》 |2012年第1期|2-6|共5页
  • 作者单位

    吉林农业大学生命科学学院,吉林长春130118;

    吉林农业大学生命科学学院,吉林长春130118;

    吉林农业大学生命科学学院,吉林长春130118;

    吉林农业大学生命科学学院,吉林长春130118;

    吉林农业大学生命科学学院,吉林长春130118;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 S642.103.5;
  • 关键词

    人参; 胡萝卜; 体细胞杂交; 杂种鉴定;

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