根癌农杆菌介导籼稻高效遗传转化体系的构建

摘要

系统分析影响根癌农杆菌介导籼稻遗传转化的主要因素,比较不同培养基、培养条件、继代次数、菌液浓度、侵染时间等因素对籼稻遗传转化的影响,并组建一个高效的籼稻遗传转化技术体系.结果表明,成熟胚或幼胚经过NMB培养基诱导,NB培养基继代培养这样的培养基组合比较适合籼稻胚性愈伤组织的培养.在农杆菌介导转化时控制好菌液浓度、侵染时间以及分化培养基中KT和NAA的浓度配比,并进行预分化处理,可显著提高抗性植株的分化效率,减少白化苗的产生,最终使转化率提高到8％.%The major factors were analyzed and compared affecting the transformation frequency of indica rice mediated by Agrobacterium including medium, culture condition, subculture frequency, bacterial concentration and infection time and so on in the paper. At last a high transformation frequency of indica rice was set up. The results indicated that NMB medium for callus inducing and NB medium for callus subculturing were the best medium combination for tissue culture of indica rice. In the meanwhile, bacterial concentration, infection time and concentration ratio of KT and NAA in differential medium at the best level were modulated. The transformed calli were pre-differentiation. So the differentiation rate was improved significantly. The albino seedlings were reduced. Finally the transformation rate of indica rice was improved to 8%.