An Agrobacterium tumefaciens-mediated efficient and stable genetic transformation reagent for strawberry

摘要

#$%^&*AU2020101957A420201001.pdf#####ABSTRACT The invention discloses an efficient and stable genetic transformation reagent for strawberry mediated by Agrobacterium tumefaciens, The formulations include: water, MS inorganic salts, B5 vitamins, sucrose ,a- lipoic acid, doxorubicin A, plant gels, antibiotics, hormones, carbenicillin, surfactants and phytosterols; Each component has a weight of 1000-1010 portions of water ,6-8 portions of MS inorganic salts and 10-12 portions of vitamins, Sucrose in 25-28 phr,a- lipoic acid in 0.0007-0.0008 phr, phytogel in 0.014-0.016 phr of doxorubicin in A, 4.5-4.7 phr, antibiotic in 5.05-5.15 phr, hormone in 0.02-0.03 phr, benzylpenicillin in 2-3 phr, surfactant in 0.1-0.2 phr and phytosterol in 0.02-0.05 phr, The reagent, Use of alipoic acid and doxorubicin A, added Has greatly improved the efficiency of genetic transformation, At the same time, the false positive rate of regenerated buds was significantly reduced, And the surfactant added to the reagent, Improved water diffusion, To quickly soak strawberries, Thus, the success rate of genetic transformation of strawberry was improved.