Components of FJAT-0809-GLX fermentation liquid inlO different initial pH values were analyzed by GC-MS. Eleven compounds with the match degree above 90 % were identified. The compounds found commonly in these 10 fermentation liquid was Hexahydro-pyrrolo [1,2-a] pyrazine-1, 4-dione. There were two sorts of functional compounds in the FJAT-0809-GLX fermentation liquids; one is volatile compounds which relate to the special aroma of this fermentation liquid, including 5-methyl-2-Furancarboxaldehyde, Maltol, Benzeneacetaldehyde, n-Hexa-decanoic acid, Oleic Acid, and 2-Hydroxy-3-methyl-2-cyclopenten-l-one. The other sort of functional compound had antiseptic and bacteriostatic effect, including Maltol, 2-Furanmethanol and Hexahydro-pyrrolo [1, 2-a] pyrazine-1,4-dione and 5-(hydroxymethyl)-2-Furancarboxaldehyde. The fermentation liquid with the initial pH value of 6 to 12 had inhibitory activity against K88. The pH value 7 had the best inhibitory activity; the diameter of inhibition zone was 20. 33mm. Different initial pH values led to different terminal pH value, and then affect the generation properties of secondary metabolites of Brevibacillus brevis FJAT-0809-GLX.%应用GC-MS对10种不同初始pH条件下发酵的FJAT-0809-GLX发酵液的丙酮萃取液进行初步成分鉴定,从这10种发酵液中得到匹配率≥90％成分11种,各发酵液中的成分存在明显差异,其中共有成分1种,为六氢吡咯并[1,2-a]吡嗪-1,4-二酮.在匹配率≥90％成分中,功能性成分按其功能可分为2类,一类为挥发性或芳香类物质,与该发酵液具有的特殊香气有关,包括5-甲基呋喃醛、2-甲基-3-羟基-4-吡喃酮、苯乙醛、棕榈酸、顺式十八碳-9-烯酸、甲基环戊烯醇酮；另一类为防腐或抑菌类成分,包括2-甲基-3-羟基-4-吡喃酮、2-呋喃甲醇、六氢-吡咯[1,2-a]吡嗪-1,4-二酮和5-羟甲基糠醛.初始pH 6～12的发酵液对大肠杆菌K88均有抑制效果,其中初始pH为7的抑菌效果最佳,抑菌圈直径达到20.33 mm.不同的初始pH影响着发酵终点pH,进而影响着短短芽孢杆菌FJAT-0809-GLX次生代谢物的产生.
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