鹤望兰二氢黄酮醇-4-还原酶基因SrDFR的克隆及表达分析

摘要

The SrDFR gene cDNA sequence involved in flavonoids synthesis was cloned from the blue petals of strelitzia reginae using RT-PCR techniques .The cDNA sequence consists of 246 bp ,encoding a polypeptide of 82 amino acids .Homology analysis showed that the deduced SrDFR protein was highly homologous to other DFR proteins from different plants . Phylogenetic analysis indicated that SrDFR was clustered together with DFR of Curcuma alismatifolia firstly and had close genetic relationship .The semi-quantitative PCR analysis indicated that SrDFR showed the highest transcript abundance in e and SrDFR was highly expressed in blue petals ,but lowly expressed in leaves and yellow sepals .The results indicated that SrDFR played an important role in the growth of blue petals .%采用RT-PCR方法从鹤望兰蓝色花瓣中克隆到类黄酮合成途径关键基因 S rD FR ，该片段长246 bp ，编码82个氨基酸。氨基酸同源性分析表明， SrDFR推导的氨基酸序列与已报道的其他植物的DFR蛋白具有很高的同源性。系统进化树分析显示，鹤望兰 S rD FR与姜荷花聚为一类，亲缘关系较近。应用半定量 PCR分析表明， S rD FR在蓝色花瓣中高表达，在黄色花萼和叶片中低表达，且在花发育的始花期表达量最高，表明该基因在蓝色花瓣发育过程中起着重要作用。