Cloning of dihydroflavonol 4-reductase gene from sweetpotato Ipomoea batatas(L.)Lam.)and its expression in E.coli

摘要

Based on the conserved sequences of genes encoding djhydroflavonol 4-reductase(DFR)from other plants,primers were designed to amplify DFR gene from sweetpotato,the gene was designate Elesignated ibDFR in this paper.The gene has 1232 bp nucleotide and encoded 398aa.Sequence comparison revealed that ibDFR sequence shared homologies of 85%,67% and 75% with those of Ipomoea purpurea,tobacco and potato.The opening read fragment of ibDFR was inserted with pET 30 a(+)using lingase to detect the expression of ibDFR in E.coli strain BL 21(DE 3).The result showed that the molecular weight of ibDFR was consistent with the calculated ones.