生防菌 TB2对甘蔗叶片抗病相关酶活的诱导作用

摘要

以多酚氧化酶（PPO）、过氧化物酶（POD）、超氧化物歧化酶（SOD）、苯丙氨酸解氨酶（PAL）和过氧化氢酶（CAT）等5种防御酶作为植物抗病性反应指标，研究生防菌 TB2和甘蔗赤腐病病菌对甘蔗植株抗病相关酶的影响。结果表明，先接病原菌后喷施生防菌 TB2、先喷施生防菌后接病原菌、病原菌处理、生防菌处理等处理后，与植物防御抗病相关的 PPO、POD、SOD、PAL、CAT 防御酶活性均比对照组高，表明 TB2和赤腐病菌均能诱导甘蔗叶片中的防御酶活性增强；先喷施生防菌后接病原菌的防御酶活性比同期先接病原菌后喷施生防菌的高，说明 TB2诱导能力强于赤腐病菌。两者共同处理的甘蔗叶片中5种防御酶活性高于单独处理。可见 TB2和赤腐病菌共同诱导具有协同增效作用。%Activities of polyphenol oxidase (PPO),peroxidase (POD),superoxide dismutase (SOD),phenylalanine ammonia lyase (PAL),and catalase (CAT)in sugarcane leaves were determined to evaluate the efficiency of a biocontrol bacterium,TB2,in inducing these disease-resisting enzymes,as well as the correlation between the enzymes and the disease resistance of sugarcane toward red rot pathogens.Treatments, including pathogen inoculation,TB2 spraying,and TB2 sprayings before and after pathogen inoculation,were applied to examine the variations on the enzymatic activities in the leaves of the treated sugarcane plants.It was found that the enzymatic activities in the treated plants were significantly higher than those in control,indicating an induction effect byeither TB2 or the red rot pathogens.The effect was greater when TB2 was applied along with the pathogenic inoculation, especiallyif the leaves were pre-sprayed with TB2.Although the biocontrol bacterium showed a stronger induction ability than the pathogens,a synergism seemed evidentwhen both were present on sugarcane.