微波辅助提取艾纳香精油的工艺优化及抗菌活性的研究

摘要

以艾纳香为原料,以艾纳香精油得率为指标,采用微波辅助水蒸气蒸馏法对艾纳香精油提取工艺进行单因素及Box-Behnken响应曲面试验进行优化,并用琼脂扩散法和琼脂稀释法对其抗菌能力进行研究.结果表明:艾纳香精油微波提取的最佳工艺条件为微波功率450W、提取时间23 min、液料比为10 (mL∶g),此条件下提取的艾纳香精油得率为3.45％,提取工艺稳定可行.5种受试菌均有抑制活性,对比抑菌圈直径和MIC,抑制顺序为黄曲霉＞黑曲霉＞金黄色葡萄球菌＞大肠杆菌＞绿脓杆菌.%This study was designed to use essental oil of Blumea balsamifera (L.) DCby microwave-assisted extraction.Effects of microwave power,extraction time and solid-liquid ratio were studied via the single factor methodologies,and then the extraction parameters was determined by Box-Behnken response surface methodology.In addition,Agar diffusion method and agar dilution method were used to see whether the extracted oil has antibacterial effect.Study results showed that optimized extraction parameters wasmicrowave power 450 W,extraction time 23 min and solid-liquid ratio 10 mL/g.An oil yield of 3.45 ％ was obtained under such conditions.Essential oil had antimicrobial activity to five kinds of microorganism.The order of antibacterial activity was aspergillus flavus＞aspergillus niger＞staphylococcus aureus＞escherichia coli＞pseudomonas aeruginosa.