琼产艾纳香叶精油的抗氧化和抗菌活性

摘要

采用水蒸气蒸馏法收集琼产艾纳香叶精油,经过GC-MS分析,精油中45种化合物被定性检出,主要是单萜类(48.55％)和倍半萜类(39.91％),相对含量列前五位的组分分别是(-)-龙脑(28.45％)、石竹烯(22.98％)、樟脑(9.56％)、α-荜澄茄烯(8.32％)和β-蒎烯(4.32％).经过DPPH自由基清除试验、β-胡萝卜素漂白试验和硫代巴比妥酸法试验发现精油具有较强的抗氧化活性.琼脂扩散法和琼脂稀释法证实精油对6种受试菌都有抑制活性,对金黄色葡萄球菌、假丝酵母和黄曲霉具有较好的抑制能力(MIC:625 μg/mL),对大肠杆菌、李斯特氏菌和沙门氏菌也表现出了抑制能力(MIC:2 500 μg/mL).以上结果证实琼产艾纳香叶精油具有明显的抗氧化和抗菌活性,拥有成为食品和化妆品的天然抗氧化剂及抗菌剂的潜力.%Essential oil of Blumea balsamifera (L.) DC.leaves cultivated in Hainan province (Qiong) was obtained by hydrodistillation.Forty-five compounds were qualitatively determined by gas chromatography-mass spectrometry (GC-MS),including monoterpenes (48.55％) and sesquiterpenes (39.91％).The top five components were (-)-borneol (28.45％),caryophyllene (22.98％),camphor (9.56％),α-cubenene (8.32％) and β-piene (4.32％).The essential oil showed antioxidant activity in DPPH radical scavenging test,β-carotene bleaching test and thiobarbituric acid reactive species assay.Agar diffusion method and agar dilution method showed that essential oil had antimicrobial activity to six kinds of microorganism.It possessed stronger inhibition capacity on Staphylococcus aureus,Candida albicans and Aspergillusflavus (MIC:625 μg/mL),and had inhibition activity on Escherichia coli,Listeria monocytogenes and Salmonella enterica (MIC:2500 μg/mL).The above results demonstrated that essential oil of Qiong Blumea balsamifera (L.) DC.leaves had significant antioxidant and antimicrobial activities,and has potential as antioxidant and antimicrobial agent used in food and cosmetic.