药物对大鼠卵巢颗粒细胞的影响

摘要

目的探讨阿米卡星、清热活血中药对雌性大鼠卵巢颗粒细胞的形态和功能影响。方法收集30日龄SD雌性大鼠卵巢颗粒细胞，分别加入不同药物进行细胞培养，并取培养液作放射免疫测定性激素，颗粒细胞作透射电镜观察细胞器。结果阿米卡星、莪术组的颗粒细胞核周见空泡，胞器基本消失，孕激素低于生理盐水组(P<0.01)，阿米卡星组的雌二醇也低于各组(P<0.05)。结论阿米卡星、莪术直接用药有碍大鼠卵巢功能。%Purpose　To explore the effects of antibiotics and herbs with removing pathogenic heat from blood on ovarian granulosa cell morphological and functions in female rats.　Methods　The granulosa cell of 30 d age SD female rats was collected,several drugs were added to the culture fluid.The culture fluid were collected to measure the sex hormone by radioimmunoassay.The cell organs of granulosa cell were measured by transmission electron microscope.　Results　Organs of the granulosa cell almost disappeared and perinuclear vesicle were founded in Amikacin sulfate group and Rhizoma zedoariae group.The progesterone levels were lower than in salline group (P<0.05).The estrogen levels of Amikacin group were lower than in saline group (P<0.05).　Conclusions　Ovarian function were obstructed in female rats when Amikacin,Rhizoma zedoariae were directly used.