高速逆流色谱分离制备胡椒中的胡椒碱

摘要

采用高速逆流色谱（high-speed countercurrent chromatography,HSCCC）法从胡椒中分离制备胡椒碱。HSCCC的溶剂系统条件为正己烷-乙酸乙酯-甲醇-水（1：1：1：1,V/V）。从5g粗提物中可一次分离得到纯度为98.72%的胡椒碱单体1.58g,分离得到的胡椒碱结构经电喷雾质谱以及核磁共振氢谱（1H NMR）和碳谱（13C NMR）进行鉴定。该法制备量大、分离效率高,对胡椒碱在食品医药领域的应用具有重要意义。%Piperine was separated from crude ethanolic pepper extract by high-speed countercurrent chromatography（HSCCC） with a solvent system composed of n-hexane-acetic ether-methanol-water（1：1：1：1,V/V）.The method allowed the separation of 1.58 g of individual piperine with a purity of 98.72% from 5 g of crude ethanolic pepper extract.The separated piperine was structurally identified by ESI-MS,1H NMR and 13C NMR.The method is of high sample loading capacity,high separation efficiency,and great importance for piperine applications in the food and medical fields.