反式脂肪酸对血管内皮细胞损伤的影响

摘要

The effects of trans-linoleic acid and trans-oleic acid on survival rate,nitric oxide synthase（NOS） activity,nitric oxide（NO） production and lactate dehydrogenase（LDH） leakage of human umbilical vein endothelial cells was studied to explore the mechanisms by which trans-fatty acids promote atherosclerosis.The results showed that both trans-fatty acids could inhibit cell proliferation through reducing NOS activity and NO secretion,increasing LDH leakage,and decreasing the vasodilation capability,thus affecting the cell oxidative stress level.Moreover,trans-linoleic acid revealed stronger cell damage than trans-oleic acid.%探讨不同反式脂肪酸促动脉粥样硬化（AS）的共同机制。通过采用反油酸和反亚油酸作用于人脐静脉内皮细胞,对其存活率、一氧化氮合酶（NOS）活性、一氧化氮（NO）生成量以及乳酸脱氢酶（LDH）渗出率的影响,比较不同反式脂肪酸对血管内皮细胞损伤的影响。结果表明：反式脂肪酸能抑制细胞增殖,可通过降低NOS活性影响NO分泌,能增加细胞LDH渗出率,从而影响细胞氧化应激水平,降低血管舒张能力。此外,实验发现反亚油酸对细胞损伤能力较反油酸强。