南极磷虾酶解工艺优化及模型建立

摘要

In the present work,alcalase was identified as the most suitable enzyme for enzymatic hydrolysis of Antarctic krill among 7 commonly used enzymes based on simultaneous consideration of chloroacetic acid-nitrogen soluble index（TCA-NSI） and degree of hydrolysis（DH）.In order to optimize the hydrolysis of Antarctic krill by alcalase,the effects of the hydrolysis conditions enzyme dosage,substrate concentration,pH,temperature and hydrolysis time on TCA-NSI and DH were studied by one-factor-at-a-time and orthogonal rotary composite design methods.Two regression models with TCA-NSI or DH as a function of each hydrolysis condition were established.The optimal process conditions for hydrolyzing Antarctic krill with alcalase were 50.7 ℃ hydrolysis temperature,pH 8.01,3010 U/g alcalase dosage and 239 min hydrolysis time.Under the optimal conditions,the TCA-NSI and DH were 73.02% and 42.33%,respectively.Meanwhile,peptides with an average length of 2.36 and a molecular mass of 277.9 were obtained.%以短肽得率（trichloroacetic acid-nitrogen soluble index,TCA-NSI）和水解度（degree of hydrolysis,DH）为指标,从7种常用酶中选出Alcalase酶作为酶解南极磷虾的最适酶。对Alcalase酶水解南极磷虾的酶用量、底物浓度、pH值、温度和时间5个因素进行单因素试验和正交旋转组合试验,建立TCA-NSI和DH与各因素的回归模型;在此基础上,结合实际生产确定Alcalase酶水解南极磷虾的最适工艺为温度50.7℃、pH8.01、加酶量3010U/g、时间239min,此时TCA-NSI值为73.02%,DH值为42.33%,短肽平均肽链长（peptide chain long,PCL）为2.36,平均相对分子质量为277.9。