Polysaccharide was extracted fromSpirulina platensis by cell freeze-thaw cooperated with hot water extraction. Operating parameters for hot water extraction and deproteinization by Sevag method of polysaccharide from cel debris after cel disruption were optimized using an orthogonal array design. The best results were obtained after extraction at 80 ℃ for 1.5 h at a solid-to-solvent ratio of 1:16 (g/mL). The extract obtained was pooled with the supernatant after cel disruption and a total total polysaccharide yield of 6.64% was obtained, which was approximately 14% higher than that obtained by hot water extraction alone. The optimal conditions for deprotenizing crude polysaccharide by Sevag’s method were found to be three treatment cycles with a mixture of chloroform and n-butyl alcohol (5:1,V/V) at a solid-to-solvent ratio of 1:3 (g/mL). Under these conditions, the deprotentinizaiton rate and the polysaccharide loss were 81.4% and 17.3%, respectively. The crude polysaccharide extract had a total sugar content of 85.3% and contained an 84700 u polysaccharide with a ploydispersity of 1.306, whereas a 54800 u polysaccharide with a ploydispersity of 2.007 was obtained by hot water extraction alone.%以螺旋藻粉为原料，将细胞冻融技术与热水浸提技术相结合提取藻多糖。通过单因素及正交试验，对冻融后破碎细胞残余物中剩余多糖的提取及Sevag法脱除蛋白进行研究，优化其工艺条件，并与传统的热水浸提工艺对比。结果表明，在料液比1：16、浸提温度80℃、浸提时间1.5h的条件下热水浸提破碎细胞残余物，效果最佳，与细胞上清液合并后的多糖总提取率达到6.64%，高于传统的热水浸提法近14%；而采用Sevag法脱除蛋白的最佳工艺条件为烷醇比5：1、料液试剂比3：1、处理次数3次。在此条件下，蛋白脱除率及多糖损失率分别为81.4%和17.3%。粗多糖质量进一步分析得，总糖含量85.3%，多糖分子质量84700u，多分散度1.306；而传统热水浸提工艺得到的多糖分子质量为54800u，多分散度2.007，其粗多糖产品质量低于细胞冻融辅助热水浸提工艺得到的产品质量。
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