不同种类氨基酸对蛹虫草菌丝体生长和虫草素含量的影响

摘要

In this study,we investigated the effect of individual addition of 20 kinds of amino acids at a concentration of 0.2 g/100 mL to liquid PDA medium for the cultivation of Cordyceps militaris in shake flasks on mycelial growth and cordycepin production.16 of the 20 kinds of amino acids had obvious mycelial growth-promoting effects on Cordyceps militaris.The top five most potent growth promoters included arginine,lysine and histidine as basic amino acids as well as aspartic acid and glutamic acid as acidic amino acids,which resulted in 2.10,1.82,1.63,1.48 and 1.40-fold increases in mycelial biomass,respectively.Moreover,each of the 20 kinds of amino acid could significantly enhance the accumulation of cordycepin in Cordyceps militaris mycelia.Addition of the basic amino acids arginine and histidine resulted in 2.91 and 2.90-fold increases in cordycepin content in Cordyceps militaris mycelia,respectively.Upon addition of the polar polar amino acids threonine and asparagine,2.43 and 2.42-fold increases,respectively,were observed,while cordycepin content in Cordyceps militaris mycelia presented a 2.05-fold increase when the non-polar amino acid alanine was added.%在蛹虫草液体摇瓶培养过程中分别添加20种不同的氨基酸,添加量为0.2g/100mL,以研究其对蛹虫草菌丝体生长和虫草素含量的影响。结果表明：在20种氨基酸中,16种氨基酸能显著促进蛹虫草菌丝体的生长。其中列前5位的依次为3种碱性氨基酸：精氨酸、赖氨酸和组氨酸,两种酸性氨基酸：谷氨酸和天冬氨酸。这5种氨基酸分别使菌丝体生长量增加了1.10、0.82、0.63、0.48、0.40倍。且每一种氨基酸添加后都能使菌丝体中虫草素含量显著增加。其中两种碱性氨基酸中的精氨酸和组氨酸添加后菌丝体中的虫草素含量分别增加了1.91和1.90倍,极性氨基酸中的苏氨酸和天冬酰胺添加后虫草素含量分别增加了1.43和1.42倍,非极性氨基酸中的丙氨酸添加后虫草素含量增加了1.05倍。