菟丝子黄酮成分分析及对食用油脂的抗氧化活性

摘要

采用紫外光谱和高效液相色谱法,对菟丝子中主要黄酮成分,即芦丁、槲皮素、山奈酚进行定性、定量分析。以芦丁为标准品,紫外光谱测定菟丝子黄酮提取物,与芦丁标准品乙醇溶液相同,在300～380nm和240～280nm有两个吸收峰,可以确定提取物属于黄酮类化合物;采用高效液相色谱法准确的测定出3种黄酮成分的含量,且重现性好,说明该方法是可靠的。以过氧化值（POV）为指标,以猪油和花生油为介质研究菟丝子黄酮的抗氧化性能。结果表明：菟丝子黄酮对食用油脂有较强的抗氧化作用,且抗氧化效果与添加量有关;在猪油和花生油中,当菟丝子黄酮添加量分别达到0.08%和0.06%时抗氧化效果已高于0.02%BHT和0.01%PG。%Flavonoids from Semen Cuscutae including rutin,quercetin and kaemperferol were determined qualitatively and quantitatively by ultraviolet spectroscopy and high performance liquid chromatography(HPLC).Using rutin as standard substance,flavonoid extract of Semen Cuscutae was determined and found to show two absorption peaks in the range of 300— 380 nm and 240 — 280 nm.The contents of three flavonoid components were determined accurately by HPLC with repeatable results.Flavonoids from Semen Cuscutae displayed strong antioxidant activity in lard and peanut oil in a dose-dependent manner as evaluated based on peroxide value.In both matrices,the antioxidant effect at doses of 0.08% and 0.06% was superior to that of 0.02% BHT and 0.01% PG.