One-factor-at-a-time method and response surface methodology were used to optimize the microwave-assisted extraction of highly deacetylated chitosan from Chirocephalus diaphanous eggshell.The optimal extraction conditions for achieving maximum degree of deacetylation of chitosan were as follows: microwave treatment time 33.4 min,ethanol soaking time 86 min,NaOH concentration 57.27%.Under these conditions,chitosan with a degree of deacetylation of 84.12% was obtained from Chirocephalus diaphanous eggshell.%以丰年虫卵壳制备的甲壳素为原料,通过单因素试验和响应面设计法,确定丰年虫卵壳壳聚糖微波辅助提取技术的最佳工艺条件为微波时间33.4min、乙醇浸泡时间86min、碱液质量分数57.27%。按此工艺条件提取可获得脱乙酰度为84.12%的丰年虫壳聚糖。
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