斑蝥黄质在雄性大鼠血清中的积累研究

摘要

This study was aimed to investigate canthaxanthin accumulation in male rat serum. A total of 38 male SD rats were selected with 250 g body weight and divided into 6 groups of 6 ones each （the remaining two served as controls）. A single （1 mL） dose ofcanthaxanthin （1.75 mg in soybean oils） was orally administered to each rat. At 0.5, 1, 2, 4, 8 h and 16 h post-administration, blood was collected from each group to analyze total flavonoids in serum by HPLC （equipped with C18 column and PDA detector）. The results obtained showed that canthaxanthin was detectable in rat serum at 0,5 h post-administration, reached the maximum level at 2 h, and at 16 h presented a level lower than that at 0.5 h.%目的：探索斑蝥黄质在雄性大鼠血清中的积累情况。方法：体质量250g雄性SD大鼠38只，随机分为6组，每组6只。每只一次灌胃1mL斑蝥黄质．豆油溶液（紫外-可见分光光度法检测斑蝥黄质含量为1．75mg／mL），剩余2只灌胃ImL食用豆油。灌胃后分别于0．5、I、2、4、8、16h各选Ⅰ组断头取血，萃取血清中总类胡萝卜素。对萃取物进行高效液相色谱（C18柱，二极管阵列检测器）分析。结果：灌胃0．5h后可在血清中检测到斑蝥黄质的信号，开始参与在大鼠消化道的消化吸收，全部消化吸收后，即2h后达到最大值。16h以后，血清中斑蝥黄质的量下降到0．5h的水平。结论：随着血液运输，斑蝥黄质几乎全部被运输至靶器官内或贮存在肝脏中。