水酶法提取榛子蛋白工艺优化

摘要

Alcalase was used for the aqueous enzymatic extraction of protein from filbert kernel.Based on one-factor-at-a-time experiments,response surface methodology was used to optimize process conditions for maximizing total protein extraction efficiency.The optimal extraction conditions were determined as follows： enzyme dosage 2.0%,hydrolysis temperature 55 ℃,hydrolysis time 2.5 h,material/liquid ratio 1：5,and hydrolysis pH of 8.9.The results of F tests showed that the process conditions could be ranked in decreasing order of contribution rate as follows： enzyme amount,hydrolysis temperature,material/ liquid ratio,pH,and hydrolysis time.%以榛子仁为原料,利用Alcalase碱性蛋白酶水解,进而提取榛子蛋白。以榛子仁总蛋白提取率为指标,对影响因素进行研究。在单因素试验的基础上采用响应面法优化工艺条件,得到最佳酶解条件：加酶量2.0%、温度55℃、酶解时间2.5h、料水比1：5（g/mL）、pH8.9。由F检验可得因素贡献率为x1〉x2〉x4〉x5〉x3,即加酶量〉酶解温度〉料液比〉酶解pH值〉酶解时间。