顶空固相微萃取-气质联用法分析单增李斯特菌污染冷藏牛肉的挥发性物质

摘要

为快速检测新鲜肉制品被单增李斯特菌污染的程度,运用顶空固相微萃取技术（head space solid-phasemicroextraction,HS-SPME）对不同污染程度单增李斯特菌的冷藏牛肉所产生的挥发性物质进行分析,确定固相微萃取法分析单增李斯特菌污染牛肉产生挥发性物质测定的条件。结果表明,单核增生李斯特菌污染牛肉产生出的挥发性物质主要有苯甲醛、十六烷、2,5-辛烷二酮、二叔丁基对甲酚、十六醛等。通过利用该方法的敏感、快速、样品用量少,操作简单、不用溶剂等优势,实现冷藏肉食品安全的在线检测需要。%Listeria monocytogenes is one of the most important food-borne pathogens causing the decay of frozen meat and listeriosis,a deadly disease.In this paper,the volatile components of frozen beef contaminated with Listeria monocytogenes during different storage periods were analyzed by headspace solid-phase microextraction（HS-SPME） coupled with gas chromatography-mass spectroscopy（GC-MS）.The results indicated that the major volatile compounds in contaminated beef were benzaldhyde,hexadecane,2,5-octanedione,butyrate and hexadecanal.The HS-SPME method has many advantages such as simple operation,fast determination,solvent free and trace sample requirement during the analytic process of flavor components in frozen beef.The method allowed the onsite detection so as to ensure the food safety of frozen meat in the modern food industry.