高效液相色谱法测定牛奶中的胆固醇

摘要

目的：建立高效液相色谱（high performance liquid chromatography,HPLC）法测定牛奶中胆固醇的方法。方法：色谱柱为Shimadzu C18柱（4.6mm×250mm,5μm）,流动相为乙腈,流速为1.0mL/min,检测波长为203nm,柱温为30℃。结果：胆固醇的线性范围为3.58～358μg/mL（r=0.9999）,平均回收率为101.8%（n=6）,相对标准偏差为1.14%。结论：该方法测定结果准确、重现性好,可作为测定牛奶中胆固醇含量的方法。%Objective： To establish an HPLC method for the determination of cholesterol in milk.Methods： Cholesterol was separated on a Shimadzu C18column（4.6 mm × 250 mm,5μm） using acetonitrile as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm and the column temperature at 30 ℃.Results： A calibration curve was established revealing a good linear relationship in the range of 3.58-358μg/mL with a correlation coefficient of 0.9999.The average spike recovery rate was 101.8% with a RSD of 1.14%.Conclusion： The method was accurate,reproducible and applicable for the determination of cholesterol in milk.