响应面法优化大球盖菇粗多糖提取工艺

摘要

The optimal extraction process for crude polysaccharides from stropharia rugoso-annulata was investigated by response surface methodology to explore the effects of extraction temperature,extraction time,extraction repeat number and solid-liquid ratio on the yield of crude polysaccharides.Based on the single-factor tests,Box-Behnken design with three key factors was conducted and a regression model was established.The results showed that optimal extraction process parameters were extraction temperature of 75 ℃,extraction time of 20 min and solid-liquid ratio of 1：25（g/mL）.Under the optimal extraction conditions,the yield of crude polysaccharides from Stropharia rugoso-annulata was up to 7.614%.%采用响应面法优化大球盖菇粗多糖提取工艺。以提取温度、提取时间及料液比作为影响大球盖菇粗多糖得率的因素,通过单因素试验选取因素与水平,根据Box-Benhnken中心组合试验设计原理,在单因素试验的基础上采用三因素三水平响应面分析法,依据回归分析确定各工艺条件的主要影响因素。结果表明：大球盖菇粗多糖水浸提的最佳工艺条件为提取温度75℃、提取时间20min、料液比1：25（g/mL）,大球盖菇粗多糖的得率达到7.614%。