动物双歧杆菌RH对消化道的耐受性及其微胶囊制备的研究

摘要

采用耐胃酸、耐胆盐、模拟胃液和模拟肠液的独立影响实验和连续模拟消化道实验考察动物双歧杆菌对消化道环境的耐受性,结果表明:动物双歧杆菌RH经各独立实验后活菌数仍保持在107 CFU/mL,经连续模拟消化道实验后活菌数为106 CFU/mL.采用单因素试验和正交试验对动物双歧杆菌RH微胶囊壁材进行优化,结果表明:动物双歧杆菌RH最佳冻干保护剂各组成质量分数分别为甘油10％、海藻酸钠0.5％、乳清蛋白1.5％、阿拉伯胶1.25％和大豆卵磷脂1.25％,该条件下制备的冻干微胶囊活菌数为1.80×1012 CFU/g,平均包埋效率为96.04％.结论:动物双歧杆菌RH对人工模拟消化道具有较好的耐受性,经壁材优化后制备的冻干微胶囊有较高的活菌数和较好的肠溶性.本研究为动物双歧杆菌RH益生菌产品的开发提供理论基础和技术支撑.%The aim of this study was to evaluate the potential of Bifidobacterium RH to be used as a probiotic product.We examined the viability of the probiotic under simulated gastrointestinal conditions and we investigated the production of probiotic microcapsules with resistance to various negative factors.The bacterial counts were 107 CFU/mL and 106 CFU/mL,respectively,under simulated gastrointestinal conditions separately and sequentially.The optimized wall material of microcasuples contained 10％ glycerol,0.50％ sodium alginate,1.50％ whey protein,1.25％ Arabic gum and 1.25％ soybean lecithin as determined by one-factor-at-a-time and orthogonal array designs.The count of viable Bifidobacterial cells in the microcapsules was 1.80 × 1012 CFU/g,and with respect to encapsulation efficiency,the results showed a mean value of 96.04％.Thus,the study demonstrated that microencapsulated Bifidobacterium RH could be resistant to simulated gastrointestinal conditions.The microencapsulation process was shown to efficiently increase the viability of probiotic cultures,and the microorganism was released from the microcapsules.This study may provide a theoretical and technical support for the development of probiotic products containing Bifidobacterium RH.