The microwave-assisted extraction of oil from Rana chensinensis eggs using anhydrous ethanol as the extraction solvent was optimized by one-factor-at-a-time method and response surface methodology.With this aim,we investigated the effect of solid-to-solvent ratio,soaking time and microwave power on the oil yield.The results obtained from the study showed that the optimized conditions were as follows:solid-to-solvent ratio,1∶9.82 (g/mL);soaking time,3.33 h;microwave irradiation time,30 min;and microwave power,600 W.Under these conditions,the oil yield could reach (20 ± 0.67)％,which was higher than that obtained by other extraction methods.By gas chromatography-mass spectrometry (GC-MS),27 fatty acids in the extracted oil were detected.Unsaturated fatty acids accounted for 54.98％ of the total fatty acids in the oil,with 9-octadecadienoic acid,cis-11,14-eicosadienoic acid,7,11,14-eicosatrienoate,8,11,14,17-eicosatetraenoate,9,12,15-octadecatrienoic acid,9-hexadecenoic acid and 7,10,13,16,19-docosapentaenoate being predominant.%以林蛙卵为原料,以无水乙醇为提取剂,研究微波法对林蛙卵油提取率的影响.在单因素试验的基础上,进行响应面优化试验,研究料液比、浸泡时间、微波功率对林蛙卵油提取的影响.结果表明,提取最佳工艺条件为料液比1∶9.82 (g/mL)、浸泡时间3.33 h、微波时间30 min、微波功率600 W;林蛙卵油的提取率可达到(20±0.67)％.与其他林蛙卵油提取方法比较,微波法辅助提取林蛙卵油具有得率高的优点.对得到的林蛙卵油采用气相色谱-质谱分析检测出27种脂肪酸,不饱和脂肪酸质量分数54.98％,其中以亚油酸、二十碳二烯酸、二十碳三烯酸、二十碳四烯酸、亚麻酸、棕榈反油酸、二十二碳五烯酸含量较高.
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