通过一步聚合法制备以双氰胺为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸为交联剂,偶氮二异丁腈为引发剂的分子印迹聚合物.通过扫描电子显微镜、红外光谱、紫外光谱等对材料的物理及化学性能进行表征.采用分子印迹固相萃取结合高效液相色谱法检测实际奶粉样品中的双氰胺残留.该方法的线性范围为0.01～2.00 mg/L,线性相关系数R2为0.999 4,呈现良好的线性关系,检出限(RSN=3)为0.57×10-2 mg/L,样品加标回收率为102.5％～104％,相对标准偏差在0.21％～0.86％之间.%A novel molecularly imprinted polymer was developed by a one-step polymerization using dicyandiamide as template,methacrylic acid as functional monomer,ethylene glycol dimethylacrylate as cross-linker,and azobisisobutyronitdle as initiator.The physicochemical properties of the prepared material were characterized by scanning electron microscopy,Fourier transform infrared spectroscopy,and ultraviolet spectroscopy.A molecularly imprinted solid phase extraction coupled with high performance liquid chromatography method was developed using this material to detect dicyandiamide in real milk powder samples.The linear range of this method was from 0.01 to 2.00 mg/L,with a correlation coefficient (R2) equal to 0.999 4,which had a good linear relationship.The limit of detection (LOD) was 0.57 × 10-2 mg/L (RSN=3).The recovery was ranged from 102.5％ to 104％,and the relative standard deviation was between 0.21％ and 0.86％.
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