Abalone muscle polysaccharide (AMP) was prepared from the muscle of Haliotis discus hannai Ino by protease hydrolysis combined with fractional precipitation with gradient concentrations of ethanol. Three fractions (AMP-1, AMP-2 and AMP-3) were obtained by DEAE-52 column chromatography. Their physicochemical properties and antitumor activity were investigated. The results of chemical composition suggested that the total sugar content of AMP was 83.9%, while the total sugar content of AMP-1 was 83.8%, which was higher than that of AMP-2 (62.5%) and AMP-3 (31.6%). Monosaccharide composition analysis suggested that AMP was mainly composed of Glc, AMP-1 was almost exclusively composed of Glc, and AMP-2 and AMP-3 were mainly composed of Glc and Gal together with hexosamine. Based on the results of chemical composition as well as ultraviolet spectroscopic and Fourier transform infrared spectroscopic analyses, AMP-1 was α-pyranose with high purity, while AMP-2 and AMP-3 might be glycoprotein. The results of iodine staining indicated that AMP-1 was a non-starch glucan. Scanning electron microscopy showed that AMP and AMP-1 had a lamellar structure, while AMP-2 and AMP-3 contained many spherical particles. Furthermore, AMP and AMP-1 showed a strong inhibitory activity against both human breast cancer cell line MDA-MB-231 and human liver cancer cell line HepG2.%利用蛋白酶酶解结合乙醇分级沉淀的方法从皱纹盘鲍肌肉中提取鲍鱼肌肉多糖(abalone muscle polysaccharide,AMP),通过DEAE-52柱层析分离后得到了AMP-1、AMP-2、AMP-3三个组分,并考察各组分的理化性质和抗肿瘤活性.化学组成的测定结果表明,AMP的总糖质量分数达到了83.9%.经DEAE-52柱层析分离后,AMP-1的总糖质量分数为83.8%,高于AMP-2(62.5%)和AMP-3(31.6%).单糖分析的结果表明,AMP主要由葡萄糖组成,而AMP-1中基本只含有葡萄糖,AMP-2和AMP-3主要由葡萄糖和半乳糖组成,同时含有较多的氨基糖.化学组成、紫外光谱和红外光谱结果表明,AMP-1是一种纯度较高的α-构型的吡喃糖,而AMP-2和AMP-3可能是糖蛋白.碘液染色结果说明AMP-1是一种非淀粉的葡聚糖.扫描电子显微镜结果显示AMP和AMP-1是片状结构,而AMP-2和AMP-3中含有较多的球状颗粒.AMP和AMP-1对MDA-MB-231细胞和HepG2细胞均表现出了良好的抑制活性.
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