为筛选东北地区采收期小麦、玉米和水稻中的真菌毒素产毒菌,以酶联免疫吸附测定、超高效液相色谱-串联质谱联用技术和聚合酶链式反应为分析工具,通过真菌分离、液体培养、产毒菌初筛、产毒性定性分析和分离菌的基因序列比对,筛选出产玉米赤霉烯酮、呕吐毒素和伏马毒素的产毒菌6 株,经DNA分析鉴定,分别属于Fusarium asiaticum、F. poae、F. graminearum和F. fujikuroi 4 种真菌.本实验结果为我国针对性的防控粮食中产毒性真菌污染提供了基础性实验数据.%This study aimed to screen out mycotoxin-producing fungal strains from wheat, corn and rice at harvest time in northeast China. ELISA, UPLC-MS/MS and PCR were adapted as analytic tools. Fungal isolation, liquid medium culture, primary screening of toxigenic strains, qualitative analysis of toxin production and gene sequence alignment of isolates were carried out. Six toxigenic strains producing zearalenone, deoxynivalenol and fumonisin were obtained and identified by DNA analysis. They were found to belong to four species, Fusarium asiaticum , F. poae, F. graminearum and F. fujikuroi. The results from this study can provide basic experimental data for the targeted prevention and control of toxic fungal contamination in foodstuffs.
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