Cu2+对多粘类芽孢杆菌增殖及其转化人参皂苷的影响

摘要

目的:研究Cu2+对多粘类芽孢杆菌增殖及转化人参皂苷的影响.方法:采用酶标仪和高效液相色谱仪分别测定加入Cu2+后多粘类芽孢杆菌的增殖及转化人参皂苷情况.结果:Cu2+促进多粘类芽孢杆菌增殖适宜质量浓度为10～50?mg/L,Cu2+质量浓度为30?mg/L时促进多粘类芽孢杆菌增殖效果最好,增殖时间最短,与对照组相比提前1?d,生物量显著增加;转化体系中Cu2+质量浓度为10～50?mg/L时,促进多粘类芽孢杆菌转化人参皂苷,Cu2+质量浓度为30?mg/L时,多粘类芽孢杆菌转化人参皂苷能力最强;但Cu2+质量浓度超过100?mg/L时反而抑制多粘类芽孢杆菌转化人参皂苷,并且随Cu2+质量浓度增大而抑制作用增强.结论:Cu2+质量浓度为30?mg/L时,可促进多粘类芽孢杆菌增殖及其转化人参皂苷.%Objective:This study aimed to examine the effect of Cu2+on the proliferation of Paenibacillus polymyxa and the conversion of ginsenosides. Methods:A microplate reader and HPLC were used for this study. Results:Cu2+at 10–50 mg/L improved P. polymyxa proliferation and the best effect was observed at 30 mg/L, where it entered the logarithmic growth phase earlier 1 d and the biomass was significantly increased as compared with the control group. The presence of Cu2+at 10–50 mg/L in the culture medium improved the conversion of ginsenosides by P. polymyxa and its effect was strongest at 30 mg/L. However, when the concentration of Cu2+exceeded 100 mg/L, the conversion of ginsenosides was inhibited, and this inhibition was increased with increasing Cu2+concentration. Conclusion:A Cu2+concentration of 30 mg/L improved P. polymyxa proliferation and the conversion of ginsenosides.