应用高通量测序技术研究新疆产区葡萄果实、叶片及果园土壤微生物多样性

摘要

利用高通量测序技术分析中国新疆酿酒葡萄产区3个葡萄园的土壤、葡萄叶片和酿酒葡萄中的微生物组成和多样性.结果表明从27个样品中共得到1 043 102个高质量真菌序列代表,包括5个菌门、237个真菌菌属;共得到2 422 188个高质量细菌序列代表,包括8个菌门、314个细菌菌属.无论是真菌还是细菌,土壤中微生物种类最为复杂、数量最多,其次是葡萄叶片和酿酒葡萄.土壤中真菌以Saccharomyces、Sordaria、Tetracladium和Geomyces为主,细菌以Arthrobacter、Kaistobacter和Skermanella为主;酿酒葡萄和葡萄叶片中真菌以Aureobasidium、Cryptococcus、Aspergillus和Sporospora为主,细菌以Pseudomonas、Sphingomonas和Adhaeribacter为主.葡萄园地理位置不同,微生物的多样性也存在差异.酿酒葡萄园复杂的微生物区系研究,对进一步开发产区特色微生物的筛选和利用提供了理论支持.%This research aimed to analyze the diversity and composition of microbial communities in soils as well as on grapevine leaves and berries from three vineyards located in Xinjiang by high-throughput sequencing.A total of 1 043 102 fungal high-quality sequences were obtained from 27 samples,representing 237 fungal genera in 5 fungal phyla,as well as 2 422 188 high-quality bacterial sequences,representing 8 bacterial phylum and 314 bacteria genus were obtained.The soil samples showed the highest diversity and quantity of both fungi and bacteria followed by grape leaves andberries.The predominant fungi identified in the soil included Saccharomyces,Sordaria,Tetracladium and Geomyces,and the predominant bacteria included Arthrobacter,Kaistobacter and Skermanella.However,for both plant materials,the predominant fungi included Aureobasidium,Cryptococcus,Aspergillus and Sporospora,and the predominant bacteria included Pseudomonas,Sphingomonas and Adhaeribacter.Moreover,microbial diversity varied from vineyard to vineyard.In summary,understanding of the complex microbial communities in the vineyard can offer a theoretical basis for further exploitation and utilization of distinctive microorganisms in the wine industry.