油茶籽油抗氧化及对自由基清除作用研究

摘要

以贵州册享农家油茶籽油为原料，添加不同浓度的茶多酚（TP）、TBHQ以及不添加为对照，贮藏在（62±2）℃的温度条件下，观察贮藏过程中酸值、过氧化值（POV）的变化；同时提取不同处理油茶籽油中多酚类物质测定贮藏前后总酚、抗氧化能力及对自由基的清除作用。结果表明：茶多酚的抗氧化效果高于TBHQ及对照组，随着添加浓度的升高而增加。高温贮藏后从油茶籽油中提取的酚类物质具有很强的抗氧化能力；对DPPH、羟基自由基和超氧阴离子自由基有良好的清除作用。其中0.02%TP＞0.03%TP＞0.04%TP＞TBHQ＞空白。%This paper regards Guizhou Ceheng farming-Camellia see d oil as raw material , adding respectively concentration to the tea polyphenol(TP) . TBHQ as the contrast with the blank group, storing at (62± 2)℃, observing the changes of acid value and peroxide value (POV), simultaneously extracting the polyphenols from the different tea seed oils observed before and after storage of total phenolic content , the antioxidant capacity and the effect of scavenging free radicals .The results show that:the antioxidant effect of tea polyphenol is better than that of TBHQ and the blank group. With the addition of tea polyphenol concentration , it increases. The phenolic substances which extracted from tea seed oil has strong antioxidant capacity after storaging at the high temperature , having better scavenging effects on DPPH , hydroxyl radical and superoxide anion free radicals , in which the effects were shown as 0.02%TP>0.03%TP>0.04%TP>TBHQ>blank.